Medwave 2016 Jun;16(5):e6471 doi: 10.5867/medwave.2016.05.6471
Retinal toxicity induced by antimalarial drugs: literature review and case report
Manuel Garza-Leon , Diana Elsa Flores-Alvarado , Juan Manuel Muñoz-Bravo
Antimalarial drugs are widely used in several countries for control of rheumatologic diseases such as systemic lupus erythematosus and rheumatoid arthritis. They are still used in Mexico because of their low cost and few secondary effects, most of which are mild and reversible. Even so, at an ophthalmological level, they could produce irreversible visual damage, which is why it is important to have ophthalmological evaluation and proper follow up. We present a clinical case as an example of characteristic ophthalmological findings as well as risk factors for retinal toxicity. We then discuss guidelines for diagnosis and follow up of patients who use antimalarial drugs for the treatment of rheumatologic illnesses.
The anti-malarial drugs, chloroquine and hydroxychloroquine, are widely known and used since their introduction in 1943 and 1955 respectively . They are frequently used as disease-modifying antirheumatic drugs, for autoimmune disorders like rheumatoid arthritis  and systemic lupus erythematosus .
Antimalarial drugs are used in Mexico and other developing countries due to their low cost and safety profile compared with second-line drugs , or in combination with other drugs . Although hydroxychloroquine is less toxic than chloroquine , the latter is used more frequently in Mexico because of their lower cost.
The main reason to stop antimalarial drugs is the profile of adverse effects associated with their use. Multiple adverse effects have been described, the most frequent are gastrointestinal (anorexia, weight loss, abdominal pain, heartburn, nausea and vomiting) (10-20%) and cutaneous (alopecia, pigmentation changes, dryness, pruritus, exanthema: exfoliative, maculopapular, urticaria, lichenoid) (10%) , most of them being mild and reversible. Damage to the retina is the most serious adverse effect of these drugs, with the risk of causing irreversible visual loss. In addition to retinal toxicity, antimalarial drugs may have other manifestations of toxicity at eye level like epithelial keratopathy with spiral pattern, subcapsular cataracts, optic nerve atrophy, and extraocular muscle paralysis with accommodation reflex palsy ,.
Although retinal toxic effects have been described even with a few months of treatment ,, in the vast majority of cases the risk increases over time, 1% after 5 to 7 years of chloroquine use . The deposit of antimalarial drugs into the retina has been described for many years; the original description of chloroquine toxicity dates back to 1959 by Hobbs et al. , while hydroxychloroquine toxicity was first described in 1967 by Scheareret et al. . Retinal toxicity incidence varies, depending on the drug, diagnostic criteria, and screening strategies used. Beinsten reported an incidence of chloroquine initial toxicity of 10% and only 0.5% of advanced retinopathy , while the incidence of toxicity from hydroxychloroquine has been reported from 0.08%  to 3.4% .
The exact mechanism by which antimalarial drugs cause retinal toxicity is not well elucidated, however, since chloroquine is a lysosomotropic agent (accumulates inside lysosomes increasing intralysosomal pH and inducing an osmotic edema with lysosomal hydrolase release) , studies in animal models suggest that the damage in lysosomal function produces an accumulation of lipofuscin ,, which is toxic to photoreceptors and retinal pigment epithelium cells (RPE). This causes degeneration of the retina similar to that described in age-related macular degeneration  or some retinal dystrophies associated with ABCA4 gene mutation . Other changes found in electron microscopy are: presence of membranous cytoplasmic bodies in ganglion, amacrine, horizontal and bipolar cells as well as autophagocytic granules accumulation in photoreceptors, primarily in the cones , with late involvement of the photoreceptors and retinal pigment epithelium . In vivo studies with optical coherence tomography (OCT) in patients who used hydroxychloroquine suggest that, in contrast to those with chloroquine use, in these patients there is primary involvement of photoreceptors their binding to the retinal pigment epithelium melanin which would prolong the toxic effects of the drug . In addition, the ganglion cells layer thickness is preserved unchanged during all the time of use, even in cases that develop retinal toxicity .
The clinical manifestations of retinal toxicity by antimalarial drugs have been well described; in early stages, changes are little evident but it is possible to observe diminished foveolar brightness or subtle pigmentary changes in both eyes. Usually during this stage, the patient remains asymptomatic or presents only minor reading difficulties; as the disease progresses, scotomas and more difficulties to read may appear since this function is done by macular cells. In the ophthalmologic examination the characteristic "bulls-eye" image can be seen, which is caused by depigmentation of the retinal pigment epithelium in the macula with a small central island spared. If exposure to the drug continues, there would be retinal pigment epithelium atrophy initially in the macular region that can progress outwards to cause retinal atrophy. This will produce a decrease in visual acuity, as well as in color and night vision. In advanced stages, funduscopic changes can resemble to those of retinitis pigmentosa with diffuse retinal changes and vascular thinning . Recently described, there is a different presentation in Asian patients in whom initial damage occurs in the extramacular region close to the vascular arcades ,.
Diagnosis, detection and follow up
Because of the severity of ocular damage associated with the use of antimalarial drugs, various associations have developed guidelines for the follow-up of these patients. One of the first and more used were the ones made by the American Academy of Ophthalmology (AAO) in 2002  which mention that there are several factors to consider, the most important is the dose used; doses greater than 400 mg/day of hydroxychloroquine or greater than 6.5 mg/kg of ideal body weight for small people, and 250 mg/day or more than 3 mg/kg of ideal body weight for chloroquine are considered risky.
In 2011, the American Academy of Ophthalmology conducted a review of these guidelines  in which it was considered that the accumulated dose (greater than 1000 g for hydroxychloroquine and more than 460 g for chloroquine) is a more appropriate parameter for monitoring. In addition to the dose, treatment duration is also an important factor; although literature describes that the risk of retinal toxicity is increased starting from five years of use, there are some risk factors for early presentation: age more than 60 years, increased body fat, kidney or liver disease, or prior macular disease . The most recent guidelines published in May 2016  agree in considering the daily dose as the most important risk factor, but focusing in patient’s real weight; They suggest that doses greater than 5.0 mg/kg of hydroxychloroquine and greater than 2.3 mg/kg of chloroquine increase the risk dramatically, in addition to the previously described duration time and tamoxifen use that increases five times the risk of toxicity.
Other risk factors not included in the American Academy of Ophthalmology recommendations, have been described only in some small studies. Palma Sanchez et al. mention systemic arterial hypertension as a risk factor . A study in Mexican patients (Araiza-Casillas et al.) found that the presence of chloroquine deposits in the cornea increases the risk of retinal toxicity five times, contrary to the general consensus of the presence of cornea verticilata caused by deposits of antimalarial drugs does not correlate with retinal toxicity, in addition of being a reversible process when stopping the medication ,.
Diagnostic approach of retinal toxicity associated with antimalarial drugs
New ophthalmology tools allow to detect early the first changes of retinopathy with multifocal electroretinography, spectral domain optical coherence tomography or fundus autofluorescence . The objective of testing asymptomatic patients is due to the fact that structural alterations precede symptoms and visual loss in advanced stages is irreversible. Until now the only treatment is the interruption of the medication. While retinal alterations can progress even after stopping the drug, progression after the interruption is much lower if diagnosed in early stages when there is no damage to the retinal pigment epithelium versus late stages .
In early stages, the only finding may be a decrease in foveal sensitivity of more than 5 dB, that will evolve to a paracentral scotoma between the two and eight degrees of the fixing point if exposure to the antimalarial drugs continues , and may progress to even more advanced scotomas.
Because of the variability of results and the strong dependence of correct performance, Amsler grid has been removed as a test method from the American Academy of Ophthalmology 2011 recommendations .
Spectral domain optical coherence tomography (SD-OCT) has also been used recently as a follow up method. In early stages, perifoveal thinning of the inner layers can be found (ganglion cells and internal plexiform layer) ,, that may progress to generalized thinning . In addition, it is possible to assess damage at photoreceptor level identified by loss of the union line of the internal and external segments  or the loss of the ellipsoid line in the parafoveal region, known as the flying saucer sign ,,. Like multifocal electroretinogram, it is possible to detect early changes in the spectral domain optical coherence tomography before noticing any alterations in the automated campimetry. Nevertheless, ring shaped scotomas can be found in 10% of cases with a normal spectral domain optical coherence . For this reason, both studies must be complemented. The sensitivity of this test has been reported to be 78.6% and specificity 98% . Besides its use for early diagnosis, spectral domain optical coherence tomography can be helpful in the follow up of patients with detected retinal toxicity, since some cases may be complicated with the development of cystoid macular edema or epiretinal membranes .
The most recent tool included for diagnostic testing is the fundus autofluorescence. It is a non-invasive diagnostic procedure that allows to evaluate lipofuscin and other fluorophores distribution in the retinal pigment epithelium. In early stages, when damage to the retinal pigment epithelium is subtle, the test can demonstrate it through a reduction in its autofluorescence, while if the damage is at photoreceptor level, there would be an increase in autofluorescence due to accumulation of debris of the outer segments ,. Like the spectral domain optical coherence tomography and multifocal electroretinogram, findings in fundus autofluorescence may precede the damage seen in the automated campimetry.
Table 1. Risks factors for chloroquine/hydroxychloroquine related retinal toxicity.
As previously said, the main risk factor for developing retinal toxicity is the cumulative dose, and because it has been estimated that prior to five years of use the risk is low, the guidelines of the American Academy of Ophthalmology suggest that annual screening should begin after five years of use in patients without other risk factors. However, a study in Mexico reported by Araiza-Casillas et al.  found that the time of chloroquine use in 16 patients with toxicity detected by automated campimetry or angiography was on average 54.1 ± 27.8 months (range 30 to 197 months), the average dose was 212.3 mg (range 122 to 272 mg) and the cumulative mean dose was less than 288 g (range of 178 to 909 g). All these values are lower than those commonly reported as risk factors, with 50% of the patients asymptomatic. Palma-Sánchez et al.  conducted another study in Spain in which they studied 40 patients using antimalarial drugs and found retinal toxicity in 13.1% (CI 95%, 5 to 21%) of them, with an average daily dose of 254 mg and cumulative mean dose of 251.3 ± 182.2 g for hydroxychloroquine and an average daily dose of 250 mg for chloroquine and a cumulative mean dose of 371.5 ± 377.2 g. We consider that it is not possible to extrapolate the recommendations described in other populations, which is why it should be discussed with the patient about the pros and cons of waiting five years for the next revision.
Currently, there is no treatment to revert the retinal damage caused by antimalarial drugs, so early diagnosis is vital to diminish the risk of serious visual loss. Once the diagnosis is made the next step is the immediate suspension of the drug. However, even after the medication is suspended, retinal toxicity can continue for several months or years in some cases. This occurs because antimalarial drugs are bind to the melanin present in pigmented tissues throughout the body  including the eyeball and are kept there even after the drug is suspended . The follow-up must be continued until having stabilization of the toxicity.
In cases in which a test reveals initial evidence of toxicity, it is recommended to repeat the studies to confirm the result and perform a careful examination to rule out other etiologies. If abnormal results are reported a second time, the patient should be informed about the risks and benefits of continuing the medication; in which case clinical follow-up and ancillary studies should be done more frequently at least every three months to evaluate for toxicity. A different treatment for the underlying disease should be discussed with the primary physician once retinal toxicity is identified.
A 56-year-old female attends to ophthalmologic consultation presenting decreased visual acuity for three years, which has been slowly progressive and gets worse in low light environments, accompanied by vision with colored halos. The patient denies eye pain, scotomas and flickering lights. She has a diagnosis of systemic lupus erythematosus for nine years, treated initially with prednisone, methotrexate (7.5 mg/week), folic acid (5 mg/day) and chloroquine (Aralen®, Sanofi-Aventis: Mexico; variable dose since the beginning, see Table 2) for six years. Prior to the start of treatment, the patient was evaluated by an ophthalmologist who did not found any contraindication for the use of chloroquine. After six years of treatment, the patient goes to the ophthalmology clinic presenting a decrease in vision. The physician suggested stopping the drug, indication commented and supported by the rheumatologist. Despite this, the patient mentions that in the past three years, decrease in visual acuity has continued.
Table 2. Patient’s weight and drug dose variation.
The ophthalmologic examination shows visual acuity of 20/200 in both eyes without improvement with stenopeic glasses, and a vision of 20/30 with lenses in both eyes. Eye movements and pupillary reflexes preserved. Intraocular pressure of 12 mmHg in the right eye and 11mmHg in left eye. In biomicroscopy, is observed in both eyes: conjunctiva with pigmented lesion in limbo, transparent cornea with retrokeratic pigment deposits and no signs of drug accumulation, deep anterior chamber with no cells and clear lens. The following signs are observed in funduscopic exam (both eyes): transparent vitreous, retina with no lesions, optic nerve with well-defined borders and central vessels and an excavation of 0.6, macula with pigmentary alteration (Figure 1a and 1b). Fundus autofluorescence (Topcon TRCNW8S Plus, Tokyo, Japan), shows a hypofluorescent lesion ring around the fovea surrounded by a hyperfluorescent area caused by debris accumulation from outer segments, known as "bulls-eye” sign (Figure 1 c and 1 d). With these findings, the diagnosis of chloroquine related macular toxicity is made.
Figure 1. Fundus photograph showing macular changes in right eye (a) and left eye (b). Autofluorescence of rigth eye (c) and left eye (d).
Auxiliary studies were conducted to determine the degree of retinal damage: 10-2 Humphrey automated campimetry with white stimulus and SITA-Standard strategy, with reliability, showing a paracentral scotoma predominantly in superior quadrants of right eye and an ring shaped central scotoma in the left eye (Figure 2a and 2b).
Figure 2. 10-2 Humphrey visual fields right eye (a) and left eye (b)
Table 2 shows the variations in drug dose and patient’s weight throughout the duration of treatment. The patient's height is 1.53 m. Accumulated dose was approximately 487g and the average weight based dose was 3.14 mg/kg. The patient did not have neither liver nor kidney damage secondary to systemic lupus erythematous, as stated by laboratory studies.
Spectral domain optical coherence tomography (CIRRUS Version 7; Zeiss Jena, Germany) with macular cube of 512 x 128 (intensity 9/10) shows areas of retinal thinning and loss of the inner/outer segment junction (high definition images, Figure 3a and 3b).
Figure 3. Optical Coherence Tomography showing effacement of the inner/outer segment junction in rigth eye (a) and left eye (b)
The patient presents classic findings of chloroquine toxicity like bull's-eye maculopathy, despite having borderline risk factors as described by the American Academy of Ophthalmology ,, given that the dose used for the first 25 months of treatment was lower than the described for toxicity and the time using a higher dose was 54 months, with a cumulative chloroquine dose of 487g (very close to the 460g suggested by the American Academy of ophthalmology). The total duration of drug use (69 months) is also very close to the 60 months (5 years) described as safe time in the guidelines. The patient’s age (56 years) is less than 60 years. The patient did not have kidney damage, there was no concomitant tamoxifen use and, according to the initial ophthalmologic exam described by the patient, there was no macular or retinal disease prior to the beginning of the treatment with chloroquine. All of this matches that described by Araiza-Casillas et al.  in Mexican patients, finding retinal toxicity even in patients without risk factors, or with borderline values, suggesting a racial predisposition or another not well studied factor that rises the need for a closer follow-up on our population.
In addition to risk factors, the patient shows findings previously described for ancillary studies that enable us to make a definitive diagnosis, like the loss of the inner/outer segment junction found in optical coherence tomography , as well as a generalized macular thinning . Autofluorescence shows alternating areas increased signal corresponding to damage at photoreceptor level with areas of decreased signal caused by damage to the retinal pigment epithelium ,. Finally, automated campimetry showed an absolute paracentral scotoma predominantly in superior quadrants of the right eye and a ring shaped scotoma in the central two to eight degrees of left eye axis.
Retinal toxicity secondary to chloroquine/hydroxychloroquine use is a condition that can cause serious and irreversible visual loss. Until today, the best treatment is early diagnosis therefore screening test should be made to all patients that use this drugs, since as exemplified in this clinical case, visual disturbances can continue even after treatment interruption.
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