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Carta a la editora
Medwave 2018 May-Jun;18(3):e7213 doi: 10.5867/medwave.2018.03.7213
Klebsiella pneumoniae productora de carbapenemasas en Perú: ¿Existe la necesidad de más estudios fenotípicos y genotípicos?
Carbapenemase-producing Klebsiella pneumoniae in Perú: Is there a need for further phenotypic and genotypic testing?
Jose Armando Gonzales Zamora
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Dear editor:

I read with interest the paper by Quispe Pari et al. about the isolation of a carbapenemase-producing Klebsiella pneumoniae in Perú [1]. The authors described the case of a 36-year-old patient from Huancayo, Perú, who was colonized with a KPC-producing Klebsiella pneumoniae. The authors used the automated system VITEK 2 for identification and antimicrobial susceptibility, which alerted them of the presence of carbapenem resistance. They also mentioned that the Kirby-Bauer method was used to confirm the production of carbapenemases. This method would be inaccurate, since the detection of carbapenemases is not possible using this technique. The Kirby-Bauer method may only evaluate for carbapenem resistance, but it does not give information about the actual mechanism of resistance, which could be secondary not only to carbapenemase, but also to porin mutations or efflux pumps [2].

For further evaluation of carbapenemases, the authors used the modified Hodge test, which is a proper test to detect most carbapenemases. However, false negatives have been reported in cases of NDM (New Delhi metallo-beta-lactamase)-producing strains and false positives in cases of mixed mechanisms of resistance different from carbapenemases [3]. Recently, many other phenotypic tests have emerged and have clearly showed higher detection rates than the modified Hodge test. Among them, the modified Carbapenem inactivation method and chromogenic tests (Carba NP, RAPIDEC CARBA NP, RAPID CARB BLUE, among others) can be mentioned. They have an overall sensitivity and specificity of 88-99% and 99-100%, respectively [4]. A clear advantage of these methods is their rapid turnaround time, which can vary from 30 minutes to 2 hours [4].

Another point of discussion is the genotypic detection of KPC reported by the authors. In their paper, they pointed out that the Klebsiella strain was tested by conventional polymerase chain reaction, but they did not mention the type of genotypic test used and no information was given regarding KPC type or gene sequence. Currently, many test for genotypic evaluation are available, such as the FilmArray BCID, the Verigene BC-GN and the X-pert Carba-R. Another test that has become very popular is the next-generation sequencing. This method sequence all the chromosomal and extrachromosomal DNA, allowing identification of genes responsible for carbapenemase, porin, and efflux pump production. This technique also give us data about strain relatedness and transmissibility. However, there is limited information about DNA sequencing of Klebsiella strains in Perú.

In Latin American context, the case from Perú published by Horna et al. disclosed the presence of a KPC-2 producing Klebsiella pneumoniae sequence type ST340 [5]. Other reports from Latin America have documented ST258 as the predominant clone. In this sense, the report of Quispe Pari et al. is an excellent initiative that will lead to further studies clearly needed in Peru to determine the epidemiology of resistant species.

Notes

Declaration of Conflict of Interest
The author declares that there is no affiliation or involvement in an organization, industry or entity with a financial or non-financial interest in the subject matter or materials discussed in this manuscript.

Financing
The author declares that there were no external sources of funding.

Licencia Creative Commons Esta obra de Medwave está bajo una licencia Creative Commons Atribución-NoComercial 3.0 Unported. Esta licencia permite el uso, distribución y reproducción del artículo en cualquier medio, siempre y cuando se otorgue el crédito correspondiente al autor del artículo y al medio en que se publica, en este caso, Medwave.

 

Autor: Jose Armando Gonzales Zamora[1]

Filiación:
[1] Division of Infectious Diseases, Department of Medicine, University of Miami, Miller School of Medicine, Miami, Florida, United States of America

E-mail: jxg1416@med.miami.edu

Correspondencia a:
[1] 1120 NW 14th Street
Suite 863B
Miami, Florida
USA
CP: 33136

Citación: Gonzales Zamora JA. Carbapenemase-producing Klebsiella pneumoniae in Perú: Is there a need for further phenotypic and genotypic testing?. Medwave 2018 May-Jun;18(3):e7213 doi: 10.5867/medwave.2018.03.7213

Fecha de envío: 5/5/2018

Fecha de aceptación: 30/5/2018

Fecha de publicación: 27/6/2018

Origen: no solicitado

Tipo de revisión: con revisión por dos pares revisores externos, a doble ciego

Ficha PubMed

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  1. Quispe Pari JF, Ingaruca Rojas JO, Castro Mucha AM, Castro Ortega ML, Ccoicca Hinojosa FJ, Montalvo Otivo R, et al. Carbapenemase producing Klebsiella pneumoniae in Peru: a case report and antimicrobial resistance discussion. Medwave. 2018 Apr 3;18(2):e7191. | CrossRef | PubMed |
  2. Dalmolin TV, Bianchini BV, Rossi GG, Ramos AC, Gales AC, Trindade PA, et al. Detection and analysis of different interactions between resistance mechanisms and carbapenems in clinical isolates of Klebsiella pneumoniae. Braz J Microbiol. 2017 Jul - Sep;48(3):493-498. | CrossRef | PubMed |
  3. Anderson KF, Lonsway DR, Rasheed JK, Biddle J, Jensen B, McDougal LK, et al. Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae. J Clin Microbiol. 2007 Aug;45(8):2723-5. Epub 2007 Jun 20. | PubMed |
  4. Tamma PD, Opene BN, Gluck A, Chambers KK, Carroll KC, Simner PJ. Comparison of 11 Phenotypic Assays for Accurate Detection of Carbapenemase-Producing Enterobacteriaceae. J Clin Microbiol. 2017 Apr;55(4):1046-1055. | CrossRef | PubMed |
  5. Villegas MV, Pallares CJ, Escandón-Vargas K, Hernández-Gómez C, Correa A, Álvarez C, et al. Characterization and Clinical Impact of Bloodstream Infection Caused by Carbapenemase-Producing Enterobacteriaceae in Seven Latin American Countries.PLoS One. 2016 Apr 22;11(4):e0154092. | CrossRef | PubMed |
Quispe Pari JF, Ingaruca Rojas JO, Castro Mucha AM, Castro Ortega ML, Ccoicca Hinojosa FJ, Montalvo Otivo R, et al. Carbapenemase producing Klebsiella pneumoniae in Peru: a case report and antimicrobial resistance discussion. Medwave. 2018 Apr 3;18(2):e7191. | CrossRef | PubMed |

Dalmolin TV, Bianchini BV, Rossi GG, Ramos AC, Gales AC, Trindade PA, et al. Detection and analysis of different interactions between resistance mechanisms and carbapenems in clinical isolates of Klebsiella pneumoniae. Braz J Microbiol. 2017 Jul - Sep;48(3):493-498. | CrossRef | PubMed |

Anderson KF, Lonsway DR, Rasheed JK, Biddle J, Jensen B, McDougal LK, et al. Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae. J Clin Microbiol. 2007 Aug;45(8):2723-5. Epub 2007 Jun 20. | PubMed |

Tamma PD, Opene BN, Gluck A, Chambers KK, Carroll KC, Simner PJ. Comparison of 11 Phenotypic Assays for Accurate Detection of Carbapenemase-Producing Enterobacteriaceae. J Clin Microbiol. 2017 Apr;55(4):1046-1055. | CrossRef | PubMed |

Villegas MV, Pallares CJ, Escandón-Vargas K, Hernández-Gómez C, Correa A, Álvarez C, et al. Characterization and Clinical Impact of Bloodstream Infection Caused by Carbapenemase-Producing Enterobacteriaceae in Seven Latin American Countries.PLoS One. 2016 Apr 22;11(4):e0154092. | CrossRef | PubMed |