VIII Congreso Internacional de Investigación REDU
← vista completaPublicado el 25 de abril de 2022 | http://doi.org/10.5867/Medwave.2022.S1.CI71
Desarrollo de nanopartículas magnéticas a lo largo de la detección de SARS-CoV-2 en Ecuador
Developing magnetic nanoparticles along the detection of SARS-CoV-2 in Ecuador
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IntroducciónThe unusual coronavirus SARS-CoV-2, which causes the COVID-19 infectious disease, struck the medical health system world wide due to its rapid person-to person propagation. Ecuador was one of the most affected Latin American Countries in april 2020, when the funeral system collapsed in the Guayaquil city due to the COVID-19 infection and the lack of detection methods at that time. A rapid and precise detection method was crucial at that time in Guayaquil/Ecuador for the detection of the SARS-CoV-2 to regulate its propagation and keep it under control. In this work we developed a facile method to synthesize magnetic nanoparticles in solution that can serve as magnetic beads produced in Ecuador allowing for the possibility of conducting around 50,000 COVID-19 tests via real time RT-PCR. Objetivosa) Synthesis of magnetic nanoparticles in solution. MétodoMagnetic nanoparticles were produced by a ’simplified three step method’ based on Zhao et al. synthesis [1] as follows: a) Coating of magnetite nanoparticles with APTES to form the amino-magnetic nanoparticles compound, b) Diacrylate-amine polymerization to obtain a Poly (ester-amine), c) Coating of the amino-magnetic nanoparticles with Poly (ester-amine) to form the final nanoparticle compound (Poly-NH2-MNP) that will be used as as magnetic beads in the real time RT-PCR procedure. Principales ResultadosIn this work, have successfully synthesized and fully characterized magnetic nanoparticles coated with Poly (ester-amine) specialized for high quality RNA extraction to identify the SARS-CoV-2 virus by real time RT-PCR. The synthesis protocol reported here serves to produce 100 mL at (10% w/v) of Poly-NH2-MNP and scaled to produce 1L of Poly-NH2-MNP (10% w/v) in two days in a basic laboratory to conduct around 50,000 real time RT-PCR tests for COVID-19 detection. By a real time RT-PCR amplification study handled by a private company in Ecuador we confirmed an increase in the fluorescence at around 38 min to confirm: i) the existence of the SARS-CoV-2 virus in the sample, and ii) the correct RNA extraction employing our Poly-NH2-MNP magnetic nanoparticles [2]. ConclusionesIn summary, we developed an optimal synthesis method for coating MNP with negatively charged polymers in the aim of the extraction and purification of the viral RNA from the SARS-CoV-2 virus. This work serves as a novel initiative along the fabrication of MNP specialized for high quality RNA extraction that can be conducted in regular laboratory facilities in developing countries, and it can be integrated into a real time RT-PCR metrology. We produced 1L of Poly-NH 2 - MNP (10% w/v) in just two days, and the efficiency of these nanoparticles were confirmed by a private company in Ecuador where we measured an increased fluorescence spectra after 38 min. characteristic of the presence of the SARS-CoV-2 virus in the sample. These results bring a key element to solving Ecuadorian budget constraints and technological developments during the existing International Public Health Emergency derived from the COVID-19. |
