VIII Congreso Internacional de Investigación REDU

← vista completa

Desarrollo de nanopartículas magnéticas a lo largo de la detección de SARS-CoV-2 en Ecuador

Developing magnetic nanoparticles along the detection of SARS-CoV-2 in Ecuador

Desarrollo tecnológico y procesos energéticos

Palabras clave
Magnetic nanoparticles, COVID-19, SARS-CoV-2, Ecuador, RT-PCR, XPS, Raman, FTIR


The unusual coronavirus SARS-CoV-2, which causes the COVID-19 infectious disease, struck the medical health system world wide due to its rapid person-to person propagation. Ecuador was one of the most affected Latin American Countries in april 2020, when the funeral system collapsed in the Guayaquil city due to the COVID-19 infection and the lack of detection methods at that time. A rapid and precise detection method was crucial at that time in Guayaquil/Ecuador for the detection of the SARS-CoV-2 to regulate its propagation and keep it under control. In this work we developed a facile method to synthesize magnetic nanoparticles in solution that can serve as magnetic beads produced in Ecuador allowing for the possibility of conducting around 50,000 COVID-19 tests via real time RT-PCR. 


a) Synthesis of magnetic nanoparticles in solution.
b) Be able to parallelize the process 10 times in just two days for delivery.
c) Proof the effectiveness of the magnetic nanoparticles in the real time RT-PCR detection method.
d) Deliver magnetic nanoparticles in solution for COVID-19 tests.
e) Support the Ecuadorian budget constraints and chemical availability during the COVID-19 International Health Emergency. 


Magnetic nanoparticles were produced by a ’simplified three step method’ based on Zhao et al. synthesis [1] as follows: a) Coating of magnetite nanoparticles with APTES to form the amino-magnetic nanoparticles compound, b) Diacrylate-amine polymerization to obtain  a Poly (ester-amine), c) Coating of the amino-magnetic nanoparticles with Poly (ester-amine) to form the final nanoparticle compound (Poly-NH2-MNP) that will be used as as magnetic beads in the real time  RT-PCR procedure.

Principales Resultados

In this work, have successfully synthesized and fully characterized magnetic nanoparticles coated with Poly (ester-amine) specialized for high quality RNA extraction to identify the SARS-CoV-2 virus by real time RT-PCR. The synthesis protocol reported here serves to produce 100 mL at (10% w/v) of Poly-NH2-MNP and scaled to produce 1L of Poly-NH2-MNP (10% w/v) in two days in a basic laboratory to conduct around 50,000 real time RT-PCR tests for COVID-19 detection. By a real time RT-PCR amplification study handled by a private company in Ecuador we confirmed an increase in the fluorescence at around 38 min to confirm: i) the existence of the SARS-CoV-2 virus in the sample, and ii) the correct RNA extraction employing our Poly-NH2-MNP magnetic nanoparticles [2].


In summary, we developed an optimal synthesis method for coating MNP with negatively charged polymers in the aim of the extraction and purification of the viral RNA from the SARS-CoV-2 virus. This work serves as a novel initiative along the fabrication of MNP specialized for high quality RNA extraction that can be conducted in regular laboratory facilities in developing countries, and it can be integrated into a real time RT-PCR metrology. We produced 1L of Poly-NH 2 - MNP (10% w/v) in just two days, and the efficiency of these nanoparticles were confirmed by a private company in Ecuador where we measured an increased fluorescence spectra after 38 min. characteristic of the presence of the SARS-CoV-2 virus in the sample. These results bring a key element to solving Ecuadorian budget constraints and technological developments during the existing International Public Health Emergency derived from the COVID-19.